Bioluminescnece imaging system for living cell and tissues
Cellgraph is an imaging system to detect low-level light emission in a single living cell by using a highly sensitive EM-CCD camera.
AB-3000B Cellgraph
Purpose and Application
- Real time reporter gene assay
- Transcriptional factor activity, Clock gene (chronopharmacology). Gene expression (Transfection,RNAi,etc)
- Analysis of cell responses
- Drug response(Anti-cancer drug,DDS,etc),Stress response (Hormone,Inflammation,Anti-Oxidant), Cytotoxichology
- Analysis of signal pathway
- Signal cascade (Calcium), Apoptosis,etc
Features
- Acquire images of culturing cells or tissue samples on a 35 mm dish in the living state.
- Provide a CO2 incubator equivalent atmosphere by controlling the temperature, CO2 gas concentration and humidifying.
- Highly sensitive and accurate detection of faint bioluminescence with an EM-CCD camera.
- Isolates and captures multicolor faint bioluminescence.
- Easy operation via a user-friendly interface.
- Full control from a PC with dedicated software.
- Completely lightproof,compact design.
Specifications
| Product Number | AB-3000B |
|---|---|
| Product Name | Cellgraph |
| Cooled CCD Camera | CCD type: Back-illuminated EMCCD |
| Active pixel: 512 x 512 pixel | |
| Pixel size: 16 x 16μm | |
| AD Resolution: 14/16 bit | |
| Cooling temperature: | |
| -80°C with an air-cooling system when the air temperature is 25°C, -90°C with a water-cooling system* | |
| (when the water temperature is 25°C) | |
| -85°C with an air-cooling system when the air temperature is 20°C, -100°C with a water-cooling system* | |
| (when the water temperature is 16°C) | |
| *The water-cooling unit is provided as an optional accessory. | |
| Objective Lens | 4x (NA 0.53) (Other magnification lenses are also available as optional accessories) |
| Stage | X-Y-Z axis manual stage, Z-axis motorized stage |
| Culture dish | 35mm culture dish |
| Constant temperature function | (Room temperature +5°C) to 45°C, 0.1°C step |
| Lighting | White LED with dimming function Blue LED with dimming function |
| Optical Filter | Up to three filters can be set (525LP/560 LP/620 LP are standard equipment) |
| Exposure time | 30 milliseconds to 90 minutes |
| Imaging interval | Indefinite |
| Control software | OS：Windows 7/Vista/XP |
| System requirement | RAM: 1 GB or more (512 MB or more for XP) |
| Hard Drive: 30 GB or more free disk space required | |
| Interface: Full size PCI slot x1, serial port x1, USB port x1 | |
| Power (main body) | AC100-240V 106VA (varies according to the components of the whole system) |
| Dimensions (main body) | 430 mm (W) x 600 mm (D) x 650 mm (H) |
| Weight (main body) | Approx. 40kg |
| Optional Accessories | |
| CCD camera water-cooling unit | Constant-temperature water circulating system, heat-resistant tube for circulation |
| CO2 gas injection unit | CO2 gas-mixer |
| CO2 gas humidifying unit | Bubbling system for gas humidification |
| Perfusion culture chamber unit | Chamber for perfusion |
| Objective lenses | 2x, 10x, 20x, 40x, 60x |